ZC BIOSCIENCE
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RAA · Recombinase-Aided Amplification

Nucleic acid results in 5–15 minutes. No thermal cycler.

ZC Bioscience develops RAA isothermal amplification reagents, rapid extraction kits and portable fluorescence analyzers for molecular testing in the lab and in the field. Reactions run at a constant 37–42 °C in lyophilised, ready-to-use tubes.

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5–15 minTime to fluorescence result
37–42 °CIsothermal, no cycling
29 SKUsReagents, kits and instruments (RUO)
8-strip reaction tubes containing lyophilised RAA reagent pellets
Lyophilised 8-strip format. Add Buffer A, Buffer B and sample DNA — then read on Genchek.
Technology

RAA — an isothermal alternative to PCR

Recombinase-Aided Amplification replaces heat-driven strand separation with enzymes, so the whole reaction runs at a single, low temperature.

RAA is a rapid isothermal nucleic acid amplification technology. A recombinase obtained from bacteria or fungi binds tightly to the primer DNA at ambient temperature, forming a recombinase–primer complex. When the primer locates its fully complementary sequence on the template, single-stranded DNA-binding protein helps open the double strand and DNA polymerase synthesises a new complementary strand. The product accumulates exponentially.

With a labelled fluorescent probe the reaction can be read in real time; a fluorescence result is typically available within 5–15 minutes. All reaction components are pre-mixed and lyophilised in the tube — simply add Buffer A, Buffer B and the extracted sample DNA.

  • 1Recombinase–primer complex scans the template and invades at the matching site.
  • 2Single-stranded DNA-binding protein stabilises the opened strands.
  • 3Strand-displacing DNA polymerase extends from the primer; new strands become templates.
  • 4Probe cleavage releases fluorescence, monitored live on the Genchek analyzer.
Illustration of a DNA double helix with two researchers
Fast

Results in 5–15 minutes — truly rapid testing, from pellet to fluorescence curve.

Isothermal

Runs at 30–42 °C; no thermal cycler or other expensive equipment required.

Accurate

High sensitivity and specificity; precise recognition of the target sequence.

Simple operation

Two-step workflow; molecular-level testing that does not require specialist staff.

Portable

Compact instruments suited to harsh field conditions and on-site inspection.

Lyophilised

Freeze-dried pellets, ready to use, easy to ship, and free from freeze–thaw loss.

RAA compared with PCR and LAMP

ParameterRAAPCRLAMP
EnzymesRecombinase, SSB, strand-displacing DNA polymeraseTaq DNA polymeraseStrand-displacing DNA polymerase
Reaction temperature37–39 °C, isothermalHigh temperature, thermal cycling60–65 °C
Reaction time15 min1.5 h40 min
Primer pairs112
Reagent formatLyophilised pelletLiquidLiquid
ReadoutGel; real-time fluorescence; lateral-flow stripGel; real-time fluorescenceVisual turbidity
Typical limitation—Slow; needs a thermal cyclerAerosol contamination, false positives

Typical values as published on the ZC Bioscience technology pages. Applications include food safety (pathogen detection, meat-species authentication, GMO screening), aquaculture pathogen detection and animal disease detection.

Products

Twenty-nine products across five categories

Core amplification chemistry, extraction, ready-made food-safety assays and the instruments that read them. All reagents are for research use only (RUO).

RAA Core Amplification Kits Genchek Analyzer Rapid Extraction Kits Food Safety Detection Series Automated System
Gloved hand holding an 8-strip of lyophilised RAA reaction tubes
Category 01 · 6 kits

RAA Core Amplification Kits (Enzyme Reagents)

Open-platform RAA chemistry for your own primers and probes. 48 tests/kit · 50 µL reaction · lyophilised · -20 °C, 12 months. Primers 30–35 bp, probes 46–52 bp. Over 10⁷-fold amplification at 30–42 °C (optimum 37–39 °C).

S001ZCRAA Basic Nucleic Acid Amplification Kit (RUO)DNA · 37 °C · agarose gel readoutRequest a Quote
S002ZCRAA Fluorescent Nucleic Acid Amplification Kit (RUO)DNA · real-time on Genchek or standard qPCR instruments · 5–20 minRequest a Quote
S003ZCRT-RAA Basic Nucleic Acid Amplification Kit (RUO)RNA · reverse transcription + RAA · gel readoutRequest a Quote
S004ZCRT-RAA Fluorescent Nucleic Acid Amplification Kit (RUO)RNA · real-time · 5–20 minRequest a Quote
S005ZCRAA-nfo Nucleic Acid Amplification Kit, Lateral-Flow Strip (RUO)DNA · 39 °C · universal nucleic acid lateral-flow strip readoutRequest a Quote
S006ZCRT-RAA-nfo Nucleic Acid Amplification Kit, Lateral-Flow Strip (RUO)RNA · 42 °C · lateral-flow strip readoutRequest a Quote
Genchek isothermal fluorescence analyzer
Category 02 · Instrument

Genchek Isothermal Fluorescence Analyzer

An integrated, portable rapid-test analyzer for on-site and mobile testing. Built-in reagent mixing, fully matched to RAA reagents; dual fluorescence channels (FAM & ROX); automatic data analysis and report generation with multiple report-export options.

Genchek Isothermal Fluorescence Analyzer8 wells × 2 channels (FAM & ROX) — up to 16 targets per run. Live fluorescence curves; all assays run under one software interface and results are read from a single display. Runs 25+ ZC Bioscience assays (RUO) across animal species, foodborne pathogens and GMO elements.Request a Quote
8 wellsThroughput, FAM / ROX
3.8 kgNet weight · 255 × 240 × 123 mm
Ambient – 65 °CTemperature range, ±0.5 °C
120 WAC 100–240 V, 50/60 Hz
5–35 °COperating, 20–80 % RH
OPENRTOSOperating system
Gloved hand holding an 8-strip of extraction tubes
Category 03 · 4 kits

Rapid Nucleic Acid Extraction Kits

Purpose-built to yield RAA-ready nucleic acid from tissue, blood, meat products, plant material, seeds and bacteria. No hazardous solvents · room-temperature storage · 12 months.

ND01ZCRapid DNA Extraction Reagent, heat-lysis (RUO)50 preps · 5 min · surfactant lysis, no organic extraction, no ethanol precipitationRequest a Quote
ND02ZCUniversal Rapid DNA Extraction Kit, spin column (RUO)50 preps · 15 min · lyse–bind–wash–elute · tissue, cells, bacteria, saliva, blood, swabs, faeces, bone, feedRequest a Quote
ND03ZCUniversal Rapid RNA Extraction Kit, spin column (RUO)50 preps · 10 min · tissue, cells, saliva, blood, swabs, faecesRequest a Quote
ND06ZCMagnetic-Bead DNA Extraction Pre-filled Plate (RUO)96 preps · 25 min · for automated magnetic-bead extractors · food safety, environmental microbiology, livestock and molecular biology researchRequest a Quote
Magnifying glass over a DNA helix
Category 04 · 17 kits

Food Safety Molecular Detection Series

Ready-to-run RAA fluorescent assays. 24 tests/kit · lyophilised · -20 °C, 6 months. Result read directly from the amplification curve — no electrophoresis. Compatible with Genchek and standard real-time PCR instruments (ABI, MJ, Roche, Bioer series).

Animal species — 10 kits LOD 1 % (w/w) · A101ZC – A110ZC
A101ZCChicken-Derived Ingredient Molecular Detection Kit, RAA fluorescent (RUO)Request a Quote
A102ZCDuck-Derived Ingredient Molecular Detection Kit (RUO)Request a Quote
A103ZCPork-Derived Ingredient Molecular Detection Kit (RUO)Request a Quote
A104ZCBovine-Derived Ingredient Molecular Detection Kit (RUO)Request a Quote
A105ZCSheep & Goat-Derived Ingredient Molecular Detection Kit (RUO)Request a Quote
A106ZCRat-Derived Ingredient Molecular Detection Kit (RUO)Request a Quote
A107ZCHorse-Derived Ingredient Molecular Detection Kit (RUO)Request a Quote
A108ZCMink-Derived Ingredient Molecular Detection Kit (RUO)Request a Quote
A109ZCFox-Derived Ingredient Molecular Detection Kit (RUO)Request a Quote
A110ZCDonkey-Derived Ingredient Molecular Detection Kit (RUO)Request a Quote
Foodborne pathogens — 7 kits LOD 10³ cfu/mL · 5–15 min · B201ZC – B207ZC
B201ZCStaphylococcus aureus Molecular Detection Kit, RAA fluorescent (RUO)Foods, cosmetics, disposable hygiene productsRequest a Quote
B202ZCSalmonella Molecular Detection Kit (RUO)FoodsRequest a Quote
B203ZCPseudomonas aeruginosa Molecular Detection Kit (RUO)Water and beveragesRequest a Quote
B204ZCListeria monocytogenes Molecular Detection Kit (RUO)Foods, including chilled foodsRequest a Quote
B205ZCHaemolytic Streptococcus Molecular Detection Kit (RUO)Foods, cosmetics, disposable hygiene productsRequest a Quote
B206ZCVibrio parahaemolyticus Molecular Detection Kit (RUO)Fish, shrimp, crab, shellfish, seaweed and other seafoodRequest a Quote
B207ZCShigella Molecular Detection Kit (RUO)FoodsRequest a Quote
GMO screening Element and event assays — enquire for availability
GMOGMO Element Detection Kits, RAA fluorescent (RUO)Bt · bar · hpt · CaMV 35S promoter · FMV 35S promoter · nos terminator; event-specific assays (soybean, maize, canola) on enquiryRequest a Quote
Product photography available on request
Category 05 · Instrument

Genchek Fully Automated Isothermal Nucleic Acid Detection System

Sample in, result out in about 30 minutes. Performs pipetting, lysis, nucleic acid purification, isothermal amplification, fluorescence detection, analysis and reporting in one instrument.

Genchek Fully Automated Isothermal Nucleic Acid Detection SystemLoad the sample and the system does the rest — extraction and amplification integrated, with real-time fluorescence detection and automatic report generation. 7-inch colour touchscreen, UV lamp and air purification built in.Request a Quote
~30 minSample-in to result-out
FAM / ROXFluorescence channels
16 kg260 × 325 × 420 mm
1 sampleThroughput per run
221 WAC 220 V, 50 Hz
>98 % accuracyPipetting, CV <5 % (<15 µL)

Catalogue numbers as listed by Hangzhou ZC Bio-Sci&Tech Co. Ltd. Instruments are laboratory equipment; all reagents and kits are for research use only.

Solutions

Three food-safety workflows, one platform

Each workflow pairs a rapid extraction step with lyophilised RAA assays read on the Genchek analyzer — about 15 minutes of amplification, no electrophoresis.

Raw meat cuts on a wooden board
Meat species authentication

Animal-derived ingredient detection

10 speciesLOD 1 % adulteration5–15 min

As price gaps between meats widen, species substitution and adulteration are increasingly common. RAA-based species kits detect species-specific genes, identifying meat origin at the molecular level with no cross-reaction observed between the listed species. Qualitative detection in animal tissue, blood, foods, meat products (incl. processed meat) and feed.

Targets
  • Chicken
  • Pork
  • Duck
  • Beef
  • Sheep / goat
  • Rat
  • Horse
  • Mink
  • Fox
  • Donkey

Users: food & drug regulators, customs and border inspection, livestock authorities, agricultural research institutes, food, supplement and feed manufacturers, third-party laboratories.

Gloved technician transferring a sample into a petri dish inside a clean bench
Microbiology

Foodborne pathogen detection

9 targetsLOD 10³ cfu/mLMulti-channel

Pathogenic bacteria account for an estimated 40–50 % of reported foodborne-illness cases in China each year. RAA pathogen kits combine multi-channel detection, short run times and high sensitivity — raising throughput and moving food-safety warnings earlier. Qualitative detection in foods, beverages, cosmetics and seafood.

Targets
  • S. aureus
  • Salmonella
  • P. aeruginosa
  • L. monocytogenes
  • Haemolytic Streptococcus
  • V. parahaemolyticus
  • Shigella
  • E. coli O157:H7
  • V. cholerae

Method reference: GB 4789 series and SN/T standards. Users: food & drug regulators, customs and border inspection, public-health laboratories, food and cosmetics manufacturers, third-party laboratories.

Blue DNA double-helix illustration
Agriculture

GMO screening

6 elementsEvent assays5–15 min

GM crops carry genes introduced from other organisms to alter traits, nutrition or quality, and regulators worldwide require labelling and control to protect consumers' right to know. RAA-based GMO kits specifically detect exogenous elements and events in crops, identifying GM material at the molecular level.

Targets
  • Bt
  • bar
  • hpt
  • CaMV 35S
  • FMV 35S
  • nos

Event assays on enquiry: soybean GTS 40-3-2, MON89788; maize MON810, Bt11; canola T45, Oxy-235. Users: regulators, agricultural research institutes, food and feed manufacturers, third-party laboratories.

Services

Support from first run to custom assay

Our R&D team answers every product question with patient explanation and demonstration, and develops RAA assays for new targets on request.

Example workflow

Animal-species assay, start to finish

RAA is simple to run and needs no specialist. Weigh the sample, extract, add two buffers and read.

  1. 1Weigh 50 mg of sample into the extraction solution; incubate at 70 °C for 10 min.
  2. 2Add 45.5 µL Buffer A, 2.5 µL Buffer B and 2 µL sample nucleic acid to the lyophilised reagent tube.
  3. 3Place in the Genchek analyzer; a result is available in 5–15 min.
Technical support

Consultation, technical documentation, training and after-sales support. Engineers respond promptly and resolve issues online or by phone; where an on-site visit is needed we dispatch an engineer and agree the arrival time with you.

After-sales service

Long-term supply of technical materials and support for every instrument and kit. Service hours Mon–Fri 9:00–18:00 (UTC+8); hotline +86 571 8861 9668.

Custom assay development

RAA is a versatile, portable rapid-testing platform that can replace LAMP or PCR in many workflows. We develop custom RAA assays for new targets and offer OEM supply — contact us to discuss your target.

Company

An R&D-driven RAA specialist in Hangzhou

Hangzhou ZC Bio-Sci & Tech Co., Ltd. ("ZC Bioscience") is an R&D-driven biotechnology company based in Hangzhou, China. We develop and apply Recombinase-Aided Amplification (RAA), an isothermal nucleic acid amplification technology, and supply RAA-based reagents, extraction kits and portable fluorescence instruments for rapid molecular testing. All reagents are for research use only.

Since September 2016 the company has accelerated product development for RAA-based rapid food-safety testing, launching in turn the animal-species molecular detection series, the foodborne pathogen series and the GMO ingredient series. Other application areas include environmental monitoring, aquaculture pathogen detection and animal disease detection.

ZC Bioscience has signed strategic cooperation agreements with government institutions and research organisations across several fields to co-develop RAA-based detection kits. We remain committed to advancing RAA technology and delivering rapid-testing products and services that are fast, accurate and easy to use.

500 m²Laboratory space, plus 400 m² of offices
RMB 12M+Installed equipment value
90 %Of the R&D team hold a master's degree or higher
5050Hangzhou Binjiang "5050 Talent Program" selection; key enterprise cultivated by the district
ZC Bioscience laboratory with technician at a workstation
Molecular laboratory, Binjiang District, Hangzhou
Technician operating laboratory equipment
Reagent production
Technician working at a clean bench
Clean-bench work
News

Notes from the field

Explainer

Recombinase-Aided Rapid Nucleic Acid Amplification

Nucleic acid amplification lets trace amounts of nucleic acid be amplified rapidly in vitro. RAA is an isothermal method in which the recombinase, single-stranded DNA-binding protein and DNA polymerase replace the heat-driven strand separation of conventional PCR, so amplification proceeds at a constant 37 °C.

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Literature

Rapid Nucleic Acid Amplification by Recombinase-Aided Amplification

Since its introduction, in-vitro nucleic acid amplification has been widely used in molecular biology and continuously improved. Recombinase-aided amplification is an isothermal method developed on the basis of existing in-vitro amplification principles.

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Highlights

RAA Technology Debuts at the Food Microbiology Detection & Control Exchange

On 20–22 July 2017 the 7th Food Microbiology Detection and Control Technology Exchange was held in Shanghai, jointly organised by the Agro-product Safety Research Center of the Chinese Academy of Inspection and Quarantine, the Analytical Microbiology Committee of the Chinese Society for Microbiology and Foodmate. The meeting ran 17 themed sessions with over 30 exhibitors and 300+ laboratory professionals.

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Forum

7th Food Microbiology Detection & Control Technology Exchange (19–22 July)

Foodborne disease caused by pathogenic microorganisms is the number-one food-safety issue. Controlling contamination, strengthening laboratory capability and applying advanced microbial detection and control technology in production have become focal concerns for regulators and food companies alike.

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Event

ZC Bioscience at the 10th China International Food Safety & Quality Conference (CBIFS 2017)

The annual CBIFS food-safety technology forum was held on 7–8 April 2017 at the Hangzhou International Expo Center. ZC Bioscience, a newcomer to food-safety testing, made its debut at the conference and its booth drew strong visitor interest.

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Contact

Request a quote or a sample run

Tell us your targets, sample types and volumes. We reply within one business day, Mon–Fri 9:00–18:00 (UTC+8).

Address
Hangzhou ZC Bio-Sci&Tech Co. Ltd
Tianhe Hi-Tech Park, Bldg 5, 688 Bin'an Rd
Binjiang District, Hangzhou 310052, China
Telephone
+86 571 8861 9668
Mon–Fri 9:00–18:00 (UTC+8)

Request a Quote

All fields help us match the right kit format and instrument.

Reagents and kits are for research use only. Not for use in diagnostic procedures.